|
KMID : 0369820120420060315
|
|
Jorunal of Korean Pharmaceutical Sciences
2012 Volume.42 No. 6 p.315 ~ p.326
|
|
|
Evaluation of mucoadhesive nanoparticle based nasal vaccine
|
|
Krishnakumar D.
Kalaiyarasi D.
Bose J. C.
Jaganathan K. S.
|
|
|
Abstract
|
|
|
|
The main objective of this study was to prepare Hepatitis B surface antigen (HBsAg) loaded poly(lactic-co-glycolic acid) (PLGA), Trimethyl chitosan (TMC) as well as TMC-coated PLGA nanoparticles and compare their efficacy as nasal vaccine. The developed formulations were characterized for size, zeta potential, entrapment efficiency, mucin adsorption ability, Dentritic cells interaction, in vitro and in vivo studies. PLGA nanoparticles demonstrated negative zeta potential whereas TMC and PLGA?TMC nanoparticles showed higher positive zeta potential. Results indicated that TMC and PLGA?TMC nanoparticles demonstrated substantially higher mucin adsorption when compared to PLGA nanoparticles. The nanoparticles were nontoxic to isolated nasal epithelium. Immunogenicity increased as a function of particle size upon nasal administration. HBsAg encapsulated in PLGA?TMC particles elicited a significantly higher secretory (IgA) immune response compared to that encapsulated in PLGA and TMC particles. Similar to in vivo immune response data, fluorescent-labelled nanoparticles of smaller size are taken up more efficiently by rat alveolar macrophages compared to those of larger size. Results indicated that alum based HBsAg induced strong humoral but less mucosal immunity. However, PLGA?TMC nanoparticles induced stronger immune response at both of the fronts as compared to generated by PLGA or TMC nanoparticles. Present study demonstrates that PLGA?TMC nanoparticles with specific size range can be a better carrier adjuvant for nasal subunit vaccines.
|
|
|
KEYWORD
|
|
|
Nanoparticles, Nasal vaccine, Hepatitis B surface antigen, PLGA, Chitosan, Trimethyl chitosan
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|